Identification of protein-protein-interactions in vitro based on high-density protein arrays

Contents 1 Introduction 1 1.1 Protein-protein-interaction screenings in vivo 2 1.1.1 Yeast two-hybrid screens 2 1.1.2 in vivo pulldown assays 4 1.1.3 Data integration and critical evaluation 6 1.2 Protein-protein-interaction screenings in vitro 6 1.2.1 Protein arrays 6 1.2.1.1 Protein microarrays 7 1.2.1.2 Arrayed cDNA expression libraries 9 1.2.2 Peptide arrays 10 1.3 Other in vitro techniques with recombinant proteins 11 1.3.1 Far Western overlay assays 11 1.3.2 in vitro pulldown assays 12